University of Regensburg Trenner Faculty of Chemistry Trenner Analytical Chemistry
Chemo- and Biosensor Group

Room: CH 12.1.82
Phone: +49-941-943-4065
FAX: +49-941-943-4064
e-mail: antje.baeumner@ur.de

Universität Regensburg
Institut für Analytische Chemie,
Chemo- und Biosensorik
93040 Regensburg, Germany

How to find us

Sekretariat:
Sabine Rudloff
Tel.: 0941-943-4066
Fax: 0941-943-4064

Antje J. Baeumner

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Germany

Since August 2013, Professor Antje Baeumner has been leading the Institute of Analytical Chemistry, Chemo- and Biosensors at the University of Regensburg in Germany. She came to Regensburg from Cornell University, where she still is an adjunct professor in the Dept. of Biological Engineering and a member of the graduate fields of Food Science and Technology, and of Biomedical Engineering.

Download CV and/or List of publications

Research

Our research adds a new emphasis on microfluidic, electrochemical, and biological sensors as well as focus on sample preparation procedures and novel nanomaterial development to the Institute. Integrated with strong expertise in optical sensing and 2D nanomaterial development already existing in our institute through the groups and research of PD Axel Dürkop and Dr. Thomas Hirsch, our four main areas of research are

Our biosensors and microfluidic systems, are novel devices that exhibit extremely high sensitivity, very high specificity and, ultimately, simplicity of use. Overlooked in the mainstream bioanalytical sensor research is the need for real-world application of the sensing systems. This is in part due to the extreme variability of sample matrices (ranging from hamburger meat to apple cider, from surface water to manure, from whole blood to saliva) and the difficulty of specifically identifying cells and proteins within these matrices. Microfluidic and fiber-based sample preparation modules are therefore part of our research efforts.

Consequently, addressing real-world challenges such as combating HIV/AIDS in resource-limited countries, providing tools for ensuring safe water and food, developing diagnostic tools for rapid and inexpensive testing are driving forces of our research. For example, biosensors we developed are capable of detecting Cryptosporidium parvum at a level of only 1 organism per sample, E. coli at 40 organisms/ml and Dengue virus at 10 pfu/ml.

Our knowledge and platform technologies include:

Liposomes

Liposomes have been developed in our research group as bioanalytical tools for signal generation and instantaneous signal amplification based on the early work in Dr. Richard Durst’s group. We have established protocols that allow for reliable liposome synthesis and for physical, chemical and biological characterization. Comparing liposomes to other signal generation and amplification means such as enzymes, quantum dots, gold nanoparticles, and fluorescence molecules had demonstrated their superior performance for bioanalytical sensors. We also study liposome stability under extreme conditions including dehydration, lyophilization, and storage at temperatures above 40 °C, in blood, urine and other matrices. We have investigated liposomes for nucleic acid, protein, and whole cell detection, and their use for bioimaging and for multiplexing. New projects investigate the development of multimodal liposomes to further enhance their bioanalytical capabilities.

For example:

Microfluidics and Sensor Miniaturization

Using fabrication techniques originally developed for microelectronics, networks of fluidic channels can be created. We employ this technology to produce small-scale devices for detection of pathogens and other analytes of interest. Our fabrication technologies include photolithography, softlithography and nanoimprinting technology. An outstanding micromill allows us to do rapid prototyping and to create complex microfluidic structures either directly in polymer substrates or within metal templates. Laser scribing is another technology we can employ for the generation of larger microchannels as well as for the development of laser-scribed graphene electrodes. These are highly porous graphitic electrodes made on polyimide foils with interesting electrochemical characteristics.

For example:

Lateral Flow Assays

Using a strip of porous membrane material as a platform, we can employ our liposome technology in the detection of target analytes in a lateral flow assay format. This format lends itself for inexpensive, rapid and extremely simple to use devices for point of care diagnostics, resource-limited settings and alike. Employing liposomes in this format allows for the visual detection of as little as 1 fmol of RNA.

For example:

Nanofibers for Analytical Applications

We develop a variety of nanomaterial-based systems including nanofibers. Electrospun nanofibers have been demonstrated by Antje Baeumner and her colleague, Margaret Frey from the Dept. of Fiber Science at Cornell University to be promising nanomaterials for bioanalytical sensors. Nanofibers can be created bearing diverse surface chemistries ranging from hydrophilic to hydrophobic or have amphoteric characteristics. They may bear functional groups, are biocompatible, non-fouling, and are capable to present biologically active molecules such as biotin on their surface. The nanofibers provide an immense surface-to-volume ratio and are therefore ideal components for integration into microfluidic devices or for paper-based microfluidics and lateral-flow assays. The Baeumner team investigates nanofibers for separation of analytes, immobilization of biorecognition elements, as detectors and also as mixing components. Dr. Nongnoot Wongkaew spearheads the development of conductive nanofibers in the institute.

For example:

Sensor Technologies

Signal amplification strategies are central to our sensor development and embrace a variety of different approaches including investigations toward highly sensitive detection strategies using electrochemical methods, electrochemiluminescence, and luminescence, as well as new materials such as upconverting nanoparticles, 2D nanomaterials and nanofibers . These find their application in the highly sensitive, multi-analyte detection of pathogens, toxins, cells and metabolites ranging from protozoan parasites like Cryptosporidium parvum to the blood clotting protein thrombin.

For example:

Available research projects:

Research projects in the above described technologies and bioanalytical sensor development areas are available at all levels. Please contact us for detailed information or simply send Dr. Baeumner an e-mail. Example project questions include the development of conductive nanofibers for electrochemical sensors, the development of novel liposome chemistries and their evaluation in bioassays, the development of electrochemiluminescence for multi-analyte detection, the development of microfluidic channel systems for bioassays, and much more.

Postdoctoral Research

Work together in a team of researchers on an independent project. Guide undergraduate or graduate students to study various aspects of your research as a small team. Contact us for possibilities and information.

Doctoral/Ph.D. Research

Work towards your Ph.D. (Dr. rer.nat.) in our vibrant research team. Participate in our intensive scientific discussions and expand your research and scientific horizon through international research stays at collaborators in Europe and the US.

Master Research (M.S. thesis and Forschungspraktikum)

Get your first experience at independent research projects. Immerse yourself in a small team of students focusing on similar challenging scientific questions and obtain experience in scientific writing, oral presentation and critical thinking.

Bachelor Research

Learn about research in a supporting and challenging environment. Work together with a graduate student or postdoc on important scientific questions related to bioanalytical sensor development and apply your class room and practical course knowledge to scientific discovery.


© 2018, Gisela Emmert.