Major Accomplishments in Bioanalytical Fluorescence
In Optical Sensor Technology
- first optical chemical sensor ever commercialized (1984)
- first 2-parameter sensor (halothane & oxygen, 1985)
- use of ion carriers in optical sensing (1986)
- use of potential-sensitive dyes in optical sensing (1986)
- first LED-based chemical sensor (1986)
- first ruthenium complexes as probes for oxygen (1986)
- first optical sensor for detection of enzyme activity (1986)
- first lifetime-based optical sensor (oxygen, 1988)
- multi-color sensor (for CO2/O2, 1988)
- microbial optical sensor (1992)
- capillary sensors (1995)
- microplates with integrated optical chemical sensors (1996; oxygen, pH)
- dual lifetime referenced (DLR) sensors (2000)
- chloride sensor for blood (2004)
- first optical chemical sensor based on upconverting nanoparticles (2009)
In Fluorescence Spectroscopy
- 2D-spectra of serum and urine, and interpretation of peaks (1983-1986)
- dual luminophore referencing (DLR, 1997) as an alternative to lifetime sensing
- modified dual DLR sensing (time domain & frequency domain) (2006)
In Imaging
- DLR imaging (2001)
- detection of skin tumor via oxygen imaging
- monitoring cell growth (chondrocytes) via imaging of pH and oxygen
- methods for imaging hydrogen peroxide, glucose, and glucose oxidase (2003 – 2005)
- simultaneous imaging of oxygen and temperature (2006)
- imaging of wound pH (2011)
In Designing Fluorescent Probes
- first chloride-sensitive probes (1982-1984)
- diode-laser excitable and specific probe for albumin (1992)
- fluorescent ruthenium probe for saccharides (1998)
- EuTc as a Eu3+-based fluorescent probe for hydrogen peroxide (2002)
- optically encoded nanobeads for bioassays (2003)
- fluorescent probes (Eu3+-based) for phosphate and phosphate esters (2004)
- terbium-based ultrasensitive DNA probe (2005)
