Research Activities by Subject
Analytical Fluorescence Spectroscopy
- measurement of decay time
- resonance energy transfer (FRET & PRET)
- photo-induced electron transfer (ion sensing)
- dual-luminophore referencing (intensity-to-phase conversion)
- single molecule detection
- fluorescence microscopy
- upconversion
- timeresolved fluorescence
Fluorescence Assays for
- ions
- gases (also dissolved)
- hydrogen peroxide
- antibodies
- oligonucleotides
- pesticides and enzyme inhibitors
- enzyme substrates incl. glucose and urea
- albumin
Fluorescent Probes
- for oxygen, pH, alkali ions, halides, H2O2
- for saccharide recognition
- probes based on photo-induced electron transfer
- µs decay time probes
- polarity-sensitive probes
- near-infrared probes
- DNA intercalators
- C70 as a probe for O2
Fluorescent Labels
- for proteins and DNAs (covalent labeling)
- diode laser-compatible labels
- with µs decay times (ruthenium lables)
- nanoparticles
- labels for electrophoresis
- labels for use in "click" chemistry
Optical Sensors (mainly fluorescent)
- for oxygen (gaseous and dissolved)
- for pH and ions (Na+, K+, Cl-)
- for gases incl. CO2, ammonia, SO2, HCl
- fiber optic sensors (20 µm - 1 mm)
- multiple sensors (O2/pH; O2/temp.; CO2/O2 etc.)
- pressure-sensitive paints (PSPs)
- planar sensors for microtiterplates and culture vials
- sensor arrays and pattern recognition
Material for Optical Sensors (polymers, plasticizers, etc.)
- polymers with permeation selectivity (hydrophilic vs. hydrophobic)
- dye-retaining polymers for sensors
- surface-reactive polymers
- molecular imprints
- polyanilines & polypyrroles
- fluorescent microbeads & nanobeads
Optical biosensors
- using immobilized enzymes
- using immobilized cells
- for substrates of oxidases (glucose etc.)
- for substrates of hydrolases (urea)
- for enzyme activity and enzyme inhibitors
Fluorescent Beads
- hydrophobic beads
- nanobeads (20 nm)
- beads with reactive surfaces
- lanthanoid nanobeads with upconversion capability (NIR to VIS)
Fluorescence Imaging Using Planar Sensors
- intensity-based
- lifetime-based
- DLR-based
- of microtiterplates
- of oxygen and pH
- of hydrogen peroxide and glucose
- of temperature
Surface and Interface Chemistry
- chemical modification of surfaces (glass, metals, organic polymers, beads)
- immobilization of dyes and biomolecules
- surface characterization (fluorescence, AFM, SFM, fluorescence microscopy)
- click chemistry reagents for use in surface chemistry
Assays Based on Lanthanoid Probes
- for hydrogen peroxide
- for peroxidase, catalase, oxidases
- substrate assays (glucose, phenol)
- EuTc-based ELISA (POx, catalase, GOx)
- gated assays (µs domain)
